Colcemid solution (10
mL bottle) --- KaryoMAX Colcemid liquid
Giemsa stain (100 mL
bottle) --- KaryoMAX Giemsa Stain Stock
Potassium chloride solution,
0.075M (about 10 mL per student) ---
KaryoMAX Potassium
Chloride Solution,
0.075M
Needed for the class:
Centrifuge
Glacial acetic acid (ACS grade)
Absolute methanol (ACS grade)
Refrigerator
Incubator (capable of
37oC)
Frosted glass slides
Needed for each person
Sterile 1 or 5 mL syringe21-guage
needle for the syringeGreen-top vacutube21-guage
multidraw needle
(for vactube)15 mL centrifuge
tube
Pasteur pipetteBulb for the pasteur pipette70
percent isopropyl alcohol pad