Sterile Petri dishes
For each Petri dish, 15
ml of CMC agar,
made with 0.5 g
carboxymethylcellulose
(a soluble form
of cellulose); 0.1 g
NaNO3
; 0.1 g K
2 HPO4 ; 0.1
g KCl;
0.05 g MgSO4
; 0.05 g yeast
extract; and
0.1 g glucose
in 100 ml of
water. The medium
should be solidified using 1.7 %
w/v agar.
Sterile water (dispensed into a McCartney bottle)
Sterile 1 ml syringes (without needles), 2
Discard beaker containing 5% solution of Chlorate I solution
Congo red solution (made with 1 mg per ml of water)
1 M sodium chloride solution
Ethanol (for flaming cork borer)
Cork borer, 5 mm diameter
Bunsen burner
Incubator set at 25-30 oC
Pen
for marking Petri dishes