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Before this crystal structure is solved, many methods are applied to the studying of SNARE structure such as electric microscope (Hans, P. I. et al. Cell, 1997) and EPR (Poirier MA, et al. Nat Struct Biol. 1998). The author has shown previously the core complex resembles the full-length complex with respect to assembly, disassembly, and biophysical properties. (Fasshauer, D. et al. JBC, 1997 Biochemistry, 1998) It was until then, the four-helix-bundle structure can be confirmed. That is, beside the two membrane bound proteins, SNAP-25 is also involved in the superhelix formation.