Cut Sites by Restriction Enzyme
EcoRI       G'AATT_C - 0 Cut(s)


HinfI       G'AnT_C - 1 Cut(s)
    152

MseI        T'TA_A - 1 Cut(s)
    168

Sau3AI      'GATC_ - 3 Cut(s)
     39    162    215


Restriction Map of Sequence 

                                           AlwI     AciI                        
                                      CviRI    Sau3AI MspA1I  Pfl1108I          
               BccI    MaeII            BsmI     DpnI      FauI                 
               \       \              \ \  \   \ \  \ \    \  \                 
      1   aaaaccatcaaatacgttatgctggaatgcaacgaactgatcccgctgttctacgaaacc     60   
          ttttggtagtttatgcaatacgaccttacgttgcttgactagggcgacaagatgctttgg          
              ^    *    ^    *    ^    *    ^    *    ^    *    ^    *          
          K  T  I  K  Y  V  M  L  E  C  N  E  L  I  P  L  F  Y  E  T            
                                                                                
                CviJI                                                           
             MspI                                                               
             ScrFI                                                              
             NciI                  Pfl1108I                                     
                BspMI          HphI             NlaIII                          
             \  \              \   \            \                               
     61   tgcccggctggtgaaaacatctgctacgaaatgttcatggttgctaccccgaaagttccg    120   
          acgggccgaccacttttgtagacgatgctttacaagtaccaacgatggggctttcaaggc          
              ^    *    ^    *    ^    *    ^    *    ^    *    ^    *          
          C  P  A  G  E  N  I  C  Y  E  M  F  M  V  A  T  P  K  V  P            
                                                                                
                                  MboII TfiI                                    
                                     MspI                                       
                             MaeII   ScrFI              MseI                    
                          TaqI       NciI         Sau3AI   BbvI     TseI        
               MaeII   CviRI    SfaNI   HinfI       DpnI      MaeII  Fnu4HI     
               \       \  \  \  \ \  \  \         \ \   \  \  \     \\          
    121   tgcgaacgtggttgcatcgacgtttgcccggaatcttctctgatcgttaaatacgtttgc    180   
          acgcttgcaccaacgtagctgcaaacgggccttagaagagactagcaatttatgcaaacg          
              ^    *    ^    *    ^    *    ^    *    ^    *    ^    *          
          C  E  R  G  C  I  D  V  C  P  E  S  S  L  I  V  K  Y  V  C            
                                                                                
                                           Sau3AI             AciI              
                         BsrI           HhaI DpnI             TauI              
              BsbI    Tsp4CI             HaeII                Fnu4HI            
           CviRI     BsiEI               AlwNI    TaqI       CviJI              
           \  \      \\  \              \\ \ \    \          \\                 
    181   tgcaacaccgaccgttgccagtaatccagcgcctgatctctcgaaataaaagccgcatt     240   
          acgttgtggctggcaacggtcattaggtcgcggactagagagctttattttcggcgtaa           
              ^    *    ^    *    ^    *    ^    *    ^    *    ^    *          
          C  N  T  D  R  C  Q  Z  S  S  A  Z  S  L  E  I  K  A  A               
                                                                                

  
Ladder Map of Restriction Enzyme Cut Sites  

                    20          40          60          80         100         120         140         160         180         200         220         240
                     :           :           :           :           :           :           :           :           :           :           :           :       
    
    HinfI ------------------------------------------------------------------------------------------\-----------------------------------------------------       
     MseI ----------------------------------------------------------------------------------------------------\-------------------------------------------       
   Sau3AI ----------------------\-------------------------------------------------------------------------\-------------------------------\---------------      


  
Pseudo-Gel Map of Digestions  

         10                                                                                                         100
          .                                .                  .            .         .        .      .      .    .    .                                .          
    HinfI                                                                                                        |                        |                           
     MseI                                                                                              |                                       |                             
   Sau3AI                                            |                    |              |                                      |